Making a Dilution Series
A potassium permanganate crystal, six large test tube , tweezers, a plastic transfer pipette, a test tube rack, 10 mL measuring cylinder.
1.Place the six test tube in a test tube rack. Lable the rack with numbers 1-6.
2.using the measuring sylinder, fill test tube 1 with 10 Ml of water. Fill the remaining test tubes with 5 mL of water.
3.Using your tweezers, add a single crystal of potassium permanganate to test tube 1.
4.Gently shake the test tube until the crystal has dissolved.
5.Using the transfer pipette, carefully remove exactly 5 mL from test tube 1 and pour it into test tube 2.
6.Rinse the transfer pipette thoroughly to ensure the no purple solution remains.
7.Gently shake test tube 2 and repeat the transfer process, transfering exectly 5 mL of solution from test tube 2 to test tube 3.
8.Rinse the pipette again and repeat the transfer process for test tube 4, 5 and 6.
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